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How do you perform a WBC estimate? Which objective do you use? What is the formula used to calculate it? What are the units?
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10x obj. # counted/5 fields x 0.15 = estimated # of WBC. units = 10^9/L
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Your WBC count should be within +/- how much % of the MaxM analyzer count?
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20%
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How do you perform a plt estimate? What obj is used? What is the formula? What are the units
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100x oil obj. # of plts counted / 5 fields x 10 = plt estimate. units = 10^9/L
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What is the formula to calculate hct? What are the units?
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HCT (L/L) = (RBC X MCV) / 1000
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What is the formula for calculating MCV? units?
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MCV (fL) = Hct/RBC x 1000
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What is the formula for calculating MCH? units?
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MCH (pg) = Hg/RBC
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What is the formula for calculating MCHC? units?
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MCHC (g/L) = Hg/Hct
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What is the formula for calculating RDW
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RDW (%) = SD/MCV x 100
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What is RDW-SD? does it depend on MCV?
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The width of the RC distn curve at the 20th percentile height; it is independent of MCV.
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In what condition would RDW-SD be high? what condition would it be low?
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High with iron deficiency anemia.
Low with thalassemia.
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Is methylene blue a basic or acidic dye? What does it stain?
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Basic dye. stains acidic (basophilic) components (like DNA)
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Is eosin a basic or acidic dye? What does it stain?
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Acidic dye. stains basic (eosinophilic) components (like some granules).
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What are the 3 ranges that are established by labs?
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Normal, Action, Critical
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What are the units for a RBC count?
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10^12/L
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What is the Electrical Impedance method aka? What kind of info does it give?
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Coulter Principle. Info on relative # of cells and cell size only.
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