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How many different ways are ATP made in bacteria?
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2 ways; substrate level phosphorylation of ADP and chemiosmotic ATP production
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How does substrate level phosphorylation occur?
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It is the direct transfer of a high energy phosphate from some compound to ADP
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How does a cell obtain the majority of its required ATP?
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Chemiosmotic ATP production, that occurs during both respiration and photosynthesis
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How does Chemiosmotic ATP production occur?
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1.Energy is stored in a Electrochemical Gradient 2.the stored energy is used to drive phosphorylation of ADP
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How do chemotrophs create an H+ gradient?
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Energy from a high energy electrons produced by oxidation of chemical compounds
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How do phototrophs create an H+ gradient?
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Photosynthetic energy captured from the light reactions of photosynthesis
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How is the proton gradient produced in mitochondria?
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H+ are pumped across the inner membrane into the intermembrane space. They diffuse through outer mem.(porins)
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How is the proton gradient produced in chloroplasts?
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Chloroplasts have internal thylakoids. Protons are pumped across the thylakoid membranes into the thylakoid space
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Graphically show how Chemiosmotic generation of ATP works in mitochondria and chloroplasts?
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Mitochondria send H+ to outside while chloroplasts send to inside vescicles.VIEW IMAGE(WITH PH)
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Where are the ATP Synthase units located in the mitochondria?
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The Inner Membrane(cristae)
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Where are the ATP Synthase units located in the chloroplast?
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The thylakoid membrane
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In what ways are the ATP synthase units reversible?
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If proton gradient exists then ADP will be phosphorlated. If not ATP will be hydrolyzed to pump H+ in intermem. space |
Where does the E to transport H+ across inner membrane come from?
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Oxidation of carbon compounds. Carbon compounds are the electron donor, and oxygen is the terminal electron acceptor
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Photosynthesis occurs in chloroplasts and the light energy captured helps with what two things?
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Production of Proton gradient and produce NADPH, the reducing agent used in the reduction of carbon
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What is the purpose of the ETC in Chemiosmotic ATP production?
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The Electron Transport chain produces the initial proton gradient
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