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Cards In This Set
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What type of coagulase is detected by the slide (latex) test method?
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Bound coag (which reacts directly with fibrinogen.)
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What type of coagulase is detected by the tube test method?
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Free coag (and also bound)
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Why do some staph aureus' appear to have a neg tube coag following incubation?
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Some staphs have fibrinolysins which are active at 35 degrees and dissolve the clot. This is why we leave the tube coags out at room temp overnight.
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What color indicates a positive DNAse result when using the toluidine blue method?
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Pink = pos
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What indicates a positive DNase result when using the HCl flood plate method?
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Clearing = pos
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What is the term used to describe infected hair follicles?
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Folliculitis
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What is the scientifc term used to describe a boil?
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Furuncle
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What is the term used to describe boils that are joined by subcutaneous sinus tracts?
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Carbuncles
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What disorder described dry, crusty lesions on the face?
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Impetigo
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What are some clinical infections associated with CNS (eg. staph epidermidis)?
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Prosthetic heart valve endocarditis, meningitis (from CSF shunts), peritonitis from dialysis, UTIs
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85% of staph aureus' are known to possess this?
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Beta-lactamases
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Susceptibilities for a bug ID'd as staph aureus show that the bug is penicillin sens? Is this accurate? What should be done before reporting the results?
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Could be true (15% of staph aureus are susceptible); perform Beta-lactamase/cefinase test to confirm.
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Which drug is used as the first choice to treat MRSA infections? What are the concerns associated with always using this drug?
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Vancomycin; concerned that VRSA will become the next superbug to take over
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What methods can be used to detect MRSA?
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SMH uses cefoxitin screen;
can use MH + 4% NaCl + oxacillin disk Can also use chromagar or molecular methods |
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Name 2 features that can differentiate micrococcus between staphs?
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Micrococcus are pigmented (yellow/red)
gram stain often shows tetrad and octad clusters (more arranged/distinct than staph clusters) micrococcus are AEROBES (not facultative ANOs) |
