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What is the Gram and microscopic arrangement of both staph and micrococcus?
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Gram +Cocci in clusters, pairs or tetrads
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Are staph and micrococcus catalase positive or negative?
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Positive
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What sites of infection are staph infections associated with?
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Various skin and mucosal surfaces when strain gains entrance to normally sterile site
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Is S. aureus coagulase negative or positive?
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Positive
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What are 3 diseases associated with Staph aureus?
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Impetigo - skin infectionScalded skin syndrome - affects babiesToxic shock syndrome - systemic
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What 3 species of Staph are assoc. with nosocomial infections other than S. aureus?
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S. epidermidisS. haemolyticusS. lugdunenis
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Are S. epidermidus, S. haemolyticus, and S. lugdunenis coag neg or pos?
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Negative
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What two characteristics of S. epidermidis enhances its likelihood of infection?
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Its production of an exopolysaccharide that enhances attachment and its ability to acquire resistance to most antimicrobial agents used in the hospital
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How is S. lugdunensis differentiated from other coag neg staph from sterile sites?
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PYR pos and pos for ornithine decarboxylase
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Why does S. lugdunensis need to be differentiated from other coag neg staph from sterile sites?
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Because it has different interpretative criteria for oxacillin
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What infection is S. saprophyticus most commonly assoc. with?
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Community acquired urinary tract infections in young sexually active females
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What media can be used to cultivate staph and micrococcus?
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5% sheep blood agar, Chocolate agar, broth blood cultures, Nutrient broths
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What selective media is used to isolate staph?
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Mannitol salt agar
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What would a colony with a yellow halo on an MSA plate indicate?
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That it is a staph colony that can ferment mannitol
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What are the incubation conditions to cultivate staph on BAP, chocolate and MSA?
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BAP and Chocolate - 35oC in CO2 or ambient air from 24 hrMSA 48-72 hr
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