SPECIAL STAINING

FOLLOWING STAINING USING A NEW BOTTLE OF EOSIN DYE POWDER, THE COLOR RESULTS ARE NOT THE SAME AS THOSE OBTAINED WITH THE PREVIOUSLY USED BOTTLE OF EOSIN DYE POWDER. THE BEST COURSE OF ACTION IS TO:

TO ACHIEVE CLARITY AND GOOD DETAIL WITH A STAINING PROCEDURE, IT IS IMPORTANT THAT THE COUNTERSTAIN BE:

IF NO CHEMICAL OXIDIZER HAD BEEN ADDED TO DELAFIELD HEMATOXYLIN, THE SOLUTION CAN BE AGED WITH:

AFTER H&E STAINING, THE CYTOPLASM IS DARK PINK AND CONTRASTS POORLY WITH THE NUCLEI. THE MOST LIKELY CAUSE IS THAT THE:

WHICH IS AN ACID COMMONLY USED TO DIFFERENTIATE EXCESS GILL HEMATOXYLIN FROM TISSUE?

FIXATION WITH THE FOLLOWING FIXATIVE WILL CAUSE IMPAIRED NUCLEAR STAINING IN SECTIONS OF AVERAGE THICKNESS

THE FOLLOWING WILL STAIN NUCLEI BLUE, BUT NO OTHER TISSUE COMPONENT:

FOLLOWING HEMATOXYLIN AND EOSIN STAINING, SLIDES ARE DEHYDRATED THROUGH ASCENDING STRENGTHS OF ALCOHOL AND CLEARED IN XYLENE. AFTER SEVERAL RACKS OF SLIDES, THE FIRST XYLENE IN THE CLEARING SERIES IS TURNING MILKY-WHITE. THE MOST LOGICAL ACTION IS TO:

MICROSCOPIC EVALUATION OF AN H&E SECTION REVEALS DARK BLUE-BLACK PRECIPITATE IRREGULARLY DISPERSED OVER THE TISSUE. THIS COULD MOST LIKELY BE CORRECTED IN THE FUTURE IF THE HEMATOXYLIN SOLUTION WAS:

A METHOD SOMETIMES USED TO INCREASE THE RATE OF STAINING IS TO:

MICROSCOPIC EXAMINATION OF AN H&E STAINED SECTION REVEALS A PINK STAINED ARTIFACT SURROUNDING THE TISSUE AND IN THE TISSUE SPACES. THE MOST PROBABLE CAUSE IS EXCESS:

MICROSCOPIC EXAMINATION OF A STAINED SLIDE REVEALS BACILLI ON, BUT NOT IN, THE TISSUE SECTIONS. THE MOST PROBABLE CAUSE OF THIS CONTAMINATION IS:

THE TYPE OF STAINING IN WHICH TISSUE BINDS THE DYE AND IS COMPLETELY PENETRATED BY THE DYE IS CALLED:

WHEN HISTOTECHS SPEAK OF "ACID DYES," THEY ARE GENERALLY REFERRING TO THE:

A COMMON ACIDIC AUXOCHROME IS: