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A number of gram positive bacteria can form a special resistant , dormant structure called
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Endospores
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Endospores develop within vegetative bacterial cells of several genera
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Bacillus and clostridium(rods), sporosarcina (cocci) ,and others
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These structures are extraordinarily resistant to environmental stresses such as
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Heat UV radiation gamma radiation chemical disinfectants and desiccation
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In fact some endospores have remained viable for around
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100,000 years
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And actinomycete spores have been recovered alive after burial in the mud for
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7,500 years
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Because of their resistance and the fact that several species of endospores forming bacteria
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Are dangerous pathogens
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Endospores are of great practical importance in
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Food industrial and medical microbiology
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This is because it is essential to be able to
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Sterilise solutions and solid objects
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Endospores often survive
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Boiling for an hour or more
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Therefore autoclaves must be used ton
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Sterilise many materials
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Endospores are also of
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Considerable theoretical interest
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In the environment, endospores aid in survival
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When moisture or nutrients are scarce
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Endospores can be examined with both
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Light and electron microscope
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Because spores are impermeable to most stains
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They are often seen as colourless area in bacteria treated with methylene blue and other simple stains
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Special spore stains are used
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To make them clearly visible
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