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Nitrogen in nitrate is much more oxidized than in
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Ammonia
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Nitrate must be first reduced to ammonia before
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Nitrogen can be converted to organic form
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Reduction of nitrate is called
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Assimilatory nitrate reduction
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It is not the same as that occurring in
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Anaerobic respiration and dissimilatory nitrate reduction
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In this nitrate is incorporated into
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Organic material and does not participate inenergy generation
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This process is widespread among
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Bacteria fungi and algae
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It takes place in the
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Cytoplasm in bacteria
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The first step is reduction to
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Nitrite by nitrate reductase, the enzyme that contains both FAD and molybdenum
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NADPH is the
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Electron source
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NO3- +NADPH + H+ ————-
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NO2- + NADP+ + H2O
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Nitrite is reduced to
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Ammonia
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With series of two electron additions catalyzed by
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Nitrite reductase and possibly other enzymes
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Hydroxylamine maybe an
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Intermediate
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Ammonia isthen incorporated into
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Amino acids
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