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Related Flashcards
Cards In This Set
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Why do you suppose a buffer was used for the isolation and function assessment of chloroplasts?
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Answer
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Why was bisulfite added to the "blank" tubes in your experiments with chloroplast function?
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Answer
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In the experiment where the amount of chloroplasts was varied, what do your results show about chloroplast function?
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Answer
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In the experiment on the effect of illumination on chloroplast function, why was it necessary to have the light on and positioned above the tube darkened with aluminum foil, since the aluminum foil should have prevented any light from reaching the chloroplasts?
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Answer
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In the reaction you have been studying in this laboratory, where are the electrons that reduce the DPIP coming from?
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Answer
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One of the most important processes in the entire biosphere is carried out by what?
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Photosynthetic Autotrophs
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What is one of the most important processes in the entire biosphere?
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Phtotosynthesis : converting light energy into useful biological enegry (ATP) and "reducing power" (CO2 to sugar by reducing Carbon to (CH2O)n, the chlorophyll-base light reactions of photosynthesis provide rge electrons and protons that reduce CO2)
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What is ATP?
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Adenosine triphosphate, a molecule that has high free energy of hydrolysis that can be coupled in many reactions to make them energetically favorable
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What absorbs the light?
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Chlorophyll
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What is the absoption spectrum?
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The pattern of absorption by a substance
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In this lab what 3 pigements did we construct an absorption spectrum for?
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Chlorophyll a, Chlorophyll b, and carotene
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What does the light energy absorbed by choloropyll permit?
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It permits the removal of electrons from water( oxidizes water) and the shuttling of electrons and protons in ways that drive the generation of ATP and to reduce CO2
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What did adding petroleum ether to the boiled spinach solution do?
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It seperated the chloropyll from the carotene into two layers
top- petroleum ether (chlorophylls a and b) bottom- ethanol (carotenoids) |
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The absorbance of light by pigments is measured by what?
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A spectrophotometer (measures from 400-700nm)
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What are the 6 parts of a spectrophotometer?
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Light source
test tube monochromator (prism of diffraction grating splits various nm and selects desired wavelength and passes that llight through test tube) light detector( converts light not absorbed into electrical signal) amplifier readout |
