Blood Collection,equipment,additives,and Order of Draw ( Florida )

Blood culture-bottles
Blue (sodium citrate )
Red (serum) electrolyte-cholesterol
Green (heparin)
Lavender (EDTA) CBC
Gray (Glycolytic Inhibitor)

Liquid portion of blood after clotting takes places
Plain red top
Tiger top

Liquid portion of blood before clotting takes place
1. Lavender top - Hemotology - ties up calcium - EDTA
2. Light blue- coagulation studies -prothrombin time and PTT. Sodium Citrate ties up Calcium.
3. Gray Top- sodium fluoride-preservatives glucose
4. Green top- used for Chemistrys. Neutralizes thrombin.
5. Yellow top -used blood cultures-contains SPS.
6. Royal blue-very seldom used-trace materials.

Supernatant

Blood vessels, platelets, coagulation factors

Hetamo=Blood
Stasis=Controlling or stopping
Hemostasis is the proceess by which the body stops the leakage of blood from the vascular system after an injury. It is the goal of the body to maintain the blood in a liquid state. Hemostasis includes the process that leads to fibrin clot formation (coagulation) as well as clot destruction (fibrinolysis). If an injury accurs to a blood vessel, the process to repair the injury is set in motion. This process is called the Cogulation Cascadeand occurs in 5 phases.

1. Vasoconstriction or Vascular
2.Platelet orPlatelet Plug Formation
3. Coagulation or fibrin clot formation
4. Clot retraction (lift off)
5. Clot destruction or fibrinolysis

Serum iron levels

Crying infants (the WBC count will return to normal in about an hour.

Acid - base balance in the body.

Some blood constitutes vary throughout the day. WBCs, eosinophil counts and iron levels are lower in the morning than in the afternoon. Cortisols are highest in the morning and are usually ordered for 8am and 4 pm for comparison.

Basal state, diet, exercise, stress, diurnal rhythms, as well as age, gender and pregnancy

Any organ that is blood forming

The ratio of additive to blood wil be incorrect.

The excess additive will cause erroneous test results.