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What is the gram rxn and microscopic arrangement of Neisseria and moraxella catarrhalis?
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Gram negative diplococci
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Are Neisseria and moraxella catarrhalis oxidase positive or negative?
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Positive
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What disease does Neisseria gonorrhoeae cause and where can it infect?
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Gonorrheacervix, conjunctiva, pharyngeal surface, anorectal area and urethra of males
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When is N. gonorrhoeae considered significant?
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It is always significant
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What diseases do N. meningitidis cause?
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Bacterial meningitis and Waterhouse-Friderichsen syndrome
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How is N. meningitidis transmitted?
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Person to person via contaminated resp. droplets
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How can N. meningitidis infection be prevented?
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Vaccine, but no vaccine against most common Group B
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What infection is N. cinerea assoc with and what can it be confused with?
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Newborn conjunctivitsconfused with N. gonorrhoeae because can be weak glucose +
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What infections are moraxella cattarhalis assoc with?
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Otitis, bronchitis, sinusitis, laryngitis, endocarditis and meningitsusually localized resp. infections
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How should specimens suspected of Neisseria and moraxella be collected?
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No cotton swabs used, inoculate directly or within 6hr to Martin Lewis agar, DO NOT REFRIGERATE!!!
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What special precaution must be taken for blood cultures suspected of Neisseria?
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SPS must not exceed 0.025% because toxic to N. gonorrhoeae and meningitidis
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What types of media are used to cultivate Neisseria and Moraxella?
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All but N. gonorrhoeae grow well on 5% sheep blood and chocolate (rest don't grow on Martin Lewis)N. gonorrhoeae requires enriched chocolate (Martin Lewis)
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What incubation conditions should be used to cultivate neisseria and moraxhella?
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3-7% CO2 at 35-37oC
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What antimicrobials are in Martin Lewis agar?
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Vancomycin - inhibits gram +colistin - inhibits gram - except NeisseriaAnisomycin or Nystatin - inhibits yeastSXT - inhibits Proteus
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What is the colonial appearance of N. gonorrhoeae on Martin Lewis?
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Gray white shiny
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