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Cards In This Set
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What is DNase?
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Extracellular enzyme that is produced within the cell and secreted outside of the cell wall
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What happens to DNase once it's secreted?
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It diffuses into the environment and breaks down macromolecular DNA molecules to the level of mononucleotide or polynucleotide
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What are nucleotides?
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Buidling blocks of amino acids
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What do nucleotide subunits serve as for bacteria?
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Nutrients
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The production and secretion of DNase has been linked to what?
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An organism's ability to degrade biofilms, which would enhance the organism's ability to invade the host or spread from the site of origin
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What is this DNase test clinically important for?
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Identification of pathogenic Staphylococcus aureus
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How is DNase agar made?
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By mixing powdered DNA into nutrient agar
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In some instances, what is added to the medium?
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Dyes such as methyl green or toluidine blue
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Does DNase agar contain dye?
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NO, it looks very much like nutrient agar
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Will all or some of the organisms used in this exercise grow on this medium?
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All
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Is the presence of DNA visible in the medium?
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No, not until the reagent hydrochloric acid is added
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When do we add HCL?
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After incubation, pour HCL onto the surface of the plate
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HCL acid is a potent what?
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Denaturant
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What wil happen as DNA in the agar denatures?
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Its structure changes and the agar will turn white wherever intact DNA is still present in the medium
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What will happen as DNA in the agar denatures?
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Its structure changes and the agar will turn white wherever intact DNA is still present in the medium
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