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Enterobacteriaceae are gram - or +?
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Gram -
Non endospore forming, facultative, anaerobic, enteric bacilli |
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Where are these groups usually found?
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Intestinal flora but can survive in soil and water
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IMVIC are how many different tests?
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4, used to identify enteric bacteria
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What does IMVIC stand for?
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Indole, methyl red, Voges-Proskauer and citrate. The I is included for pronounciation purposes
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What is used to detect indole production?
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SIM medium along with Kovac's reagent
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How is Indole produced?
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From the enzymatic hydrolysis of the amino acid tryptophan
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Organisms that produce tryptophanase metabolize tryptophan to produce what 3 things?
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Indole, Pyruvic acid and ammonia
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SIM stands for what?
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Sulfide, indole and motility
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This medium contains what 2 things?
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Iron salt and Thiosulfate
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What happens when thiosulfate is produced?
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H2S gas produced reacts with the iron salt in the medium to form a black iron sulfide precipitate (H2S positive)
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SIM contains ___ which provides the organism with amino acids?
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Peptone
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To determine if the organims produces tryptophanase what do you do?
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The medium is first inoculated and incubated. After incubation, add Kovac's reagent directly to the surface of the agar in the tube. If the reagent turns cherry red color, indole was produced. If the reagent does NOT change color and remains a pale yellow, the culture is indole-Negative
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Is this medium solid or semisolid?
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Semisolid
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What color is Kovac's reagent?
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Red
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The concentration of agar in SIM medium is about what percent of the agar concentration of a traditional agar?
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1/5
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