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Describe the appearance of staphylococci on a smear? (gram +/- and what arrangement)
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Gpc, pr, cl
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Describe the appearance of micrococci on a smear? (gram +/- and what arrangement, size)
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Gpc, TETRADS!!! (this distinguishes micrococci from staphylococci), they are also larger than staph.
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Describe the appearance of staphylococci on BAP (poss colors, poss hemolyses)
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Cream, golden, white, BH or NH, op
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Describe the appearance of micrococci on BAP (color)
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Stable pigment, can be carotenoid (yellow or red) esp at lower temps
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What kind of atmosphere do staph. require? (ie. what are they in terms of oxygen/carbon tolerance/use)
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Facultative ANAEROBE
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What kind of atmosphere do micrococci require? (ie. what are they in terms of oxygen/carbon tolerance/use)
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AEROBES
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What are the results for staph when a lysostaphin and modified oxidase test are performed?
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Lysostaphin = Sensitive
MO = Negative
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What are the results for micrococci when a lysostaphin and modified oxidase test are performed?
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Lysostaphin = Resistant
MO = Positive
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Are staph considered pathogenic? Are micrococci considered pathogenic?
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Staph are pathogenic.
Micrococci are not usually.
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Describe the appearance of staph. aureus on BAP (size, hemolysis, color)
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Med-lg, BH (NH) golden, cr, wh
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What are the results for TC, SC, DNase and Novobiocin tests when run on staph. aureus; which is the most important test for this organism?
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TC pos, SC pos, DNase pos, Novobiocin S
TC is the most important 'gold standard'
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Describe the appearance of staph. epidermidis on BAP (hemolysis, size, color)
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NH (BH), sm-med, wh-gr
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Describe the appearance of staph. saprophyticus on BAP (hemolysis, size, color)
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NH, lg, chalk-wt, possibly yellow or orange
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What are the results for staph. epidermidis when TC, SC, DNase, and novobiocin tests are run?
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TC neg, SC, neg, DNase neg, Novobiocin Sens
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What are the results for staph. saprophyticus when TC, SC, Dnase, and novobiocin tests are run?
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TC neg, SC neg, DNase neg, Novobiocin Resistant
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