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Plate count Dilution factor formula: vol moved x DF moved % total volOCD formula: #CFU%DF of plate counted |
Aerotolerance Oxygen requirements for growthbrain heart infusion agar used Obligate Anaerobe: Growth on top onlyFacultative Org: growth Top-middle-bottomInf in a puncture wound: Aerotolerant bacteriaMicrophile requires a small concentration of oxygen for growth. |
Macconkey Agarselective and differential mediaselective agent: Crystal violet ad bile saltdifferential agent: LactoseGram- are inhibited in this mediaUsed for Dx purposeThis media detects coliforms but do not differentiate them,When POSITIVE, the Org are surrounded by a Hot pink haloThe pink indicates acidic conditions from the fermentation of LactoseThe pH in this media is neutral RED which turns pik in acidic conditions. |
Plate count |
Negative stain |
Mannitol Salt Agar Selective and differential mediaUsed for DX Pathogenic S. AureusThe Org surrounded by yellow is considered positive The yellow represente the acidic waste products from the fermetation of a carbohydrate.The dark pink represente the alkaline wate products from the breakdown of preteins The selective agent is NaClThe differntial agent is MANNITOLBacteria that are capable to grow in this media are non-halophilic and gram negatives |
Capsule stain |
Acid-FAst stain |
Endospore stain |
Euglena |
Hanging drop
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Anabaena |
Spirogyra |
Piridinium |
Diatoms |
Saccharomyces serevisiae |
Paramecium |
Rivularia
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Chlamydomonas
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Oscillatoria
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