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Cards In This Set
| Front | Back |
 Detect hydrogen sulfide gas production |
Peptone iron agar deep containing ferrous sulfate(+) Black precipitate(-) No change
|
 Detects urease production; useful in distinguishing Proteus |
Urea broth, phenol red(+) Fuchsia/ magneta(-) Yellow/ orange
|
 Test for lysine and ornithine decarboxylase (one way to overcome IMViC tests limit.- p.63) |
Lysine & Ornithine broth, mineral oil, Bromcresol purple(+) Purple(-) No color change/ yellow
|
 Test for phenylalanine deaminase - most bacteria that produce this are pathogenic (ie Proteus, Morganella |
Phenylalanine slants, 10% ferric chloride(+) Green color on surface of medium(-) No color change
|
 Detect ability to utilize citrate acid as sole source of carbon |
Simmon's citrate agar, bromthymol blue(+) medium turns from green to deep blue(-) No color change; remains dark green
|
 Detects the presence of acetonin |
MR-VP broth, Barritt's reagent(+) Deep pink(-) No color change
|
 Ability to ferment glucose and form acids |
MR-VP broth, Methyl Red(+) Red(-) Yellow
|
 Test for Ability to Break down Tryptophan and yield indole |
Tryptone broth, Kovac's reagent(+) Red color on surface of broth(-) Yellow
|
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Test for Catalase Production
|
Hydrogen peroxide(+) Bubbling(-) No bubbling
|
 Detect cytochrome c oxidase activity (All Enterobacteriacease are negative) |
TMPD(+) Purple(-) Colorless
|
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Identify the way nitrate is reduced in certain bacteria
|
Nitrate reagent A&B, Zinc(+) Red after adding reagents A &B; if added zinc- colorless(-) Remained red after adding zinc
|
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Detect if bacteria can ferment different carbs
|
Durham tubes, Fermentation broth + carb (glucose, lactose, sucrose),
phenol red(+) Yellow(-) Red
|
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Detect if bacteria can break down carbs (C. diphtheriae, C.
perfringes, B. anthracis)
|
Starch agar, Iodine(+) Clear zone around bacterial growth when iodine is added(-) Purple color after iodine added
|
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Detect if bacteria can break down tributyrin
|
Tributyrin agar(+) Clear zone around bacterial growth(-) No clear zone
|
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Detect if bacteria can break down gelatin (S. aureus, L.
monocytogenes
|
Nutrient gelatin(+) failure to solidify at cold temp(-) Solid at cold temp
|
