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Cards In This Set
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You should flame a tube
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before and after putting your loop in.
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You use a quadrant streak plate to get
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isolation from two or more species in a mixed culture.
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Give me one term used to describe the whole colony, margin, or elevation in terms of colonial morphology
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Undulate(wavy)
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What does NB/NA/TSA stand for?
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Nutrient Broth, Nutrient Auger, and Triptic Soy Auger
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What objective should you always start with on the microscope?
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The scanning objective which is the lowest powered objective on the microscope.
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After making a bacterial smear it must be:
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heat fixed before staining and viewing in order to kill cells and adhere them to the slide.
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Name one of the stains we will be using today:
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Methylene Blue, Safranin, Crystal Violet, Nigrosin, Malachite Green
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Name the technique we will be using today to determine if bacteria is motile:
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Hanging Drop
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What do we use to decolorize a Gram Stain?
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Alcohol or Acetone
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What does a differential stain do?
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Allows a microbiologist to detect differences between organisms or differences between parts of the same organism These differences are often structural.
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A spore is located terminally in a cell where is it located?
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At the end of the cell
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How long do you stain with Malachite Green in an endospore stain or Carbolfuchion in an acid fast stain?
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5 minutes
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Name one of the organisms that either doesn’t require oxygen or is not adversely affected by it:
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Facultative Anaerobe or Aerotolerant Anaerobe
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Name 2 of the 4 techniques used to assess oxygen requirements of a microorganism:
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Agar Shake/ Agar Deep Stab/ Thioglycollate tube/ Anaerobic jar with a gas pack
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In what part of a test tube with media is the oxygen content the lowest?
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The bottom
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