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 Identify organisms 1 - 5 as aerobic or anaerobic. be as specific as possible |
1 = strict aerobe, 2 = strict anaerobe, 3 = facultative anaerobe, 4 = microaerophile, 5 = aerotolerant anaerobe
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What is a moderate anaerobe? Give 3 examples of spps that are moderate anaerobes.
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Can survive in 2-8% O2, but doesn't grow. B. fragilis, C. perfringens, Fusobac nucleatum
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Give an example of a strict anaerobe.
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Clostridium novyi
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What is an aerotolerant anaerobe? Give an example of 1.
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Can survive & grow in reduced O2 conditions, but growth is ltd. Preference is for ANO2. P. acnes
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What is a microaerophile? Give an example.
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Is not really an anaerobe, it requires O2 but at reduced concentrations. Campylobacter jejuni
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Give 3 examples of diseases assoc w/ exogenous infections by anaerobic bacteria
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Food poisoning, gangrene (myonecrosis), diarrhea (CDAD)
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Give 3 examples of comon diseases assoc w/ endogenous infections by anaerobic bacteria
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Abscesses (breast, soft tissue, cutaneous), dental infections, intra-abdominal or pelvic sepsis
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Why aren't anaerobes commonly assoc w/ bacteremia or sinus infections?
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Too much O2 in these environments.
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What temperature should collected samples be stored at if anaerobic infection is suspected? Why at this temp?
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RT. Refrigeration enhances oxygenation, lose the recovery of anaerobes.
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What is the 2 hr lab rule for anaerobic bacteria?
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Collected sample must be planted w/in 2 hrs of collection to ensure recovery of anaerobes.
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Anaerobic infections (particularly abscesses) can have the following 4 properties.
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Foul smell or exudate; lots of gas; sulphur granules; infection located near mucosal surface
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Are anaerobes resistant or susceptible to metronidazole?
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Susceptible
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Which ingredients are added to BRU to 'enrich' it?
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Na bisulfate, hemin, vitamin K
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Which spp demonstrates classic double zone hemolysis on BRU?
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Clostridium perfringens
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Which spp is pigmented brown to black with a foul odor on BRU?
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Prevotella or Porphomonas
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