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- Yes
- Citrobacter freundii
- Enterobacter aerogenes
- Escherichia coli
- Proteus vulgaris
- Salmonella typhimurium
- Pseudomonas
- No
- Staphylococcus aureus
- Streptococcus durans
- Micrococcus luteus
- Neisseria sicca
- Klebsiella pneumoniae
- Shigella sonnei
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- The iron in media reacts with H2S --> dark precipitate
- +
- Proteus vulgaris
- Citrobacter freundii
- Salmonella enteriditis
- some Edwardsiella
- -
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- Tryptophanase production?
- tryptophan --> NH3+pyruvate+indole
- +
- Escherichia coli
- Edwardsiella tarda
- Providencia stuartii
- Proteus vulgaris
- -
- Enterobacter aerogenes
- Salmonella enteriditis
- Acinetobacter baumannii
- Listeria innocua
- Sarcina aurantica
- Sporosarcina ureae
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- citrate as sole carbon source
- can it produce citrate permease?
- +
- Enterobacter cloacae
- Citrobacter freundii
- Pseudomonas aeruginosa
- Providencia stuartii
- Acinetobactter baumannii
- -
- Escherichia coli
- Edwardsiella tarda
- Alcaligenes faecalis
- Listeria innocua
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- differentiate between lactose and non-lactose fermenters
- +
- Escherichia coli
- Klebsiella pneumoniae
- Citrobacter freundii
- Proteus vularis
- Providencia stuartii
- Enterobacter aerogenes
- Serratia marcescens
- -
- Salmonella enteriditis
- Shigella sonnei
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- produce urease?
- +
- Proteus vulgaris
- Providencia stuartii
- Sporosarcina ureae
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- pyruvate broken down to mixed acids
- lactic acid, succinic acid, ethanol acetic acid
- Reagent: Methyl Red Reagent
- +
- Escherichia coli
- Proteus vulgaris
- Salmonella enteriditis
- Providencia stuartii
- Edwardsiella tarda
- Morganella morganii
- Hafnia alveoli
- Pseudomonas aeruginosa
- -
- Shigella sonnei
- Citrobacter freundii
- Klebsiella pneumoniae
- Yersinia, Acinetobacter, Listeria, Sporosarcina, Alcaligenes, Sarcina, Enterobacter
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LDC (lysine decarboxylase)
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- +
- Enterobacter aerogenes
- Edwardsiella tarda
- Salmonella enterditis
- Citrobacter freundii
- Pseudomonas aeruginosa
- Acinetobacter baumannii
- -
- Proteus, Providencia. Shigella, Alcaligenes, Morganella, Bacillus, Listeria, Sporosarcina, Sarcina
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ODC (ornithine decarboxylase)
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- +
- Edwardsiella tarda
- Salmonella enterditis
- Citrobacter freundii
- Pseudomonas aeruginosa
- Shigella sonnei
- Morganella morganii
- Acinetobacter baumannii
- -
- Proteus, Providencia, Alcaligenes, Bacillus, Listeria, Sporosarcina, Sarcina
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- pyruvate converted to acetoin (end prod) + CO2
- butanediol fermentation
- Reagents: A (alpha-napthol) and B (KOH)
- +
- Enterobacter aerogenes
- Serratia marcescens
- Erwinia carotovora
- -
- Escherichia coli
- Pseudomonas aeruginosa
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- can reduce nitrate to nitrite in anaerobic condition
- Reagents: A (sulfanilic acid) & B (dimethyl-alpha-napthylamine)
- color change after AB addn --> (+)
- clear after zinc addn --> (+)
- clear but color change after zinc addn --> (-)
- +
- Escherichia coli
- Edwardsiella tarda
- Morganella morganii
- Pseudomonas aeruginosa
- Sprosarcina ureae
- -
- Alcaligenes faecalis
- Acinetobacter baumannii
- Listeria innocua
- Sarcina aurantica
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- growth in salt environment?
- +
- -
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- phenol red indicator change color when acid is produced --> lower pH
- yellow indicates that sugar was fermented and acid was produced
- red indicates no sugar fermentation
- Durham tube --> gas production
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- produce amylase?
- Reagent: iodine
- if no amylase, starch should be present and form complex with iodine (negative result) --> dark
- if positive for amylase production, iodine has no starch to form complex with --> clear zone around growth
- +
- Bacillus cereus
- Staphylococcus aureus
- -
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- caseinase production?
- +
- Bacillus cereus
- Staphylococcus aureus
- Micrococcus luteus
- -
- Alcaligenes faecalis
- Acinetobacter baumannii
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