Prepare for Microbiology Final Practical with Flashcards

Prepare for Microbiology Final practical briefly with these flashcards and revise the concepts. Learn the essential terms, definitions, and much more briefly with these flashcards quizzes.

25 cards | Total Attempts: 188 | Created by PaulaTherriault | Last updated: Jan 22, 2021 | Total Attempts: 188

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Prepare for Microbiology Final Practical with Flashcards - Flashcards

Cards In This Set

Front	Back
	Positive CAMP Test Arrowhead zone of clearing, Streptococcus agalactiae acts with beta-hemolysin of Staph aureus
	DNA Hydrolysis Clearing in agar DNAse present + No clearing DNAse absent -
	SIM Sulfur reduction Left tube H2S negative Right tube H2S positive
	Urea broth Pink rapid urea hydrolysis; strong urease production + Orange or yellow No urea hydrolysis; organism does not produce urease or cannot live in broth -
	Decarboxylation No color change no decarboxylation -Yellow Fermentation; no decarboxylation -Purple Decarboxulation; specific decarboxylase enzyme +Removes carboxyl amino group
	Vogues-ProskauerRed + butanediol fermentation aceoin produced +No color change butanediol fermentaion acetoin is not produced +
	Oxidation/Fermentation Mineral oil added for anerobic Green or blue Oxidation O Yellow Oxidation and fermentation or fermentation only O-F or F Slight yellow at top Oxidation and slow fermentation or slow fermentation O-R or F Green or blue No sugar metabolism; organism is nonsaccharolytic N
	Catalase Bubbles catalase +No bubbles Catalase -
	Gas(non-fermenter) Denitrification-production of NO3 to NO2 N2 Gas fermenter or status unknown Requires additional reagents Red after reagents A and B Nitrate reduction to nitrite NO3 to NO2 No color change after reagents A and B Incomplete-add zinc No color after zinc Nitrate reduction to nongaseous nitrogenous compounds Red after zinc No nitrate reduction
	IndoleKovac reagentRed ring indole +No red indole -
	Gamma hemolysis
	Beta hemolysis
	Alpha hemolysis
	Citrate utilizationBlue Citrate is utilized +No color change; growth citrate is utilized +No color change, no growth citrate is not utilized -
	Oxidase Dark blue/purple cytochrome c oxidase is present No color change in 20 sec Cytochrome c oxidase not present