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Cards In This Set
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TRNA 3' terminal site for amino acid attachment
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Aminoacyl tRNA synthetase
aminoacyl= any amino acid synthetase is specific for each A.A
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D-Loop contains
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Dihydrouridine (DHU loop located near the 5' end)
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C-T Loop contains
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Pseudouridine- same structure as uridine but the ribosome is not attached to the nitrogen
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Extra arm
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Invovled in aminoacyl tRNA synthetase recognition (variable arm)
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3' end of tRNA
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Amino acid accepter, ester link from 3'-OH (CCA end) to the COOH of an amino acid
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What determines the specificity of the genetic code
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TRNAs
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Wobble
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TRNA can recognize >1 codon
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Degeneracy
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Number of codons for a single amino acid
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FMet (start) list codes, degeneracy, and number of tRNA
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AUG, GUG, UUG, 3degrees, and 1 tRNA
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Stop
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UGA, UAR, 3 degrees, and 0 tRNA since stop codons are not translated (start codons are)
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Shine-Dalgarno Sequence
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AGGAGG sequence found before the start Codon AUG helping the ribsome mount mRNA for translation. Not absolutely needed not in postive gram prokaryotes but only found in prokaryotes.
Mutations in S-D can reduce translation
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Suppressor tRNAs
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Suppress mutations that lead to early termination
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Amber mutations/ Amber suppressor
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Changes normal codon to stop codon UAG/ suppressor allows a stop codon to have an amino acid set into the protein
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Formation of fMet-tRNAfmet
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TRNAfMet--> Met-tRNAfmet--> modified to fMet-tRNAfMet
N-formyl is a aldehyde attached to the methionine Nitride
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S unit
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Used as a measure for time it takes to centerfuges
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