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Cards In This Set
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What is a mutation?
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A heritable change in DNA nucleotide base sequence of the gemone that can lead to a change in teh genotypes as well as phenotype
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How does a mutant necessarily differ from its parental strain?
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In GENOTYPE (not necessarily phenotype)
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What can a growth advantage under a specific set of conditions be used for in genetic engineering?
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A selectable mutation
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Give examples of selectable and non selectable mutations.
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Selectable - antibiotic resistance
Nonselectable - pigmentation |
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How would you scan for a non-selectable mutant?
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Screen by simply examining large numbers in order to identify the mutant
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How do you detect an auxotroph?
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Inability to grow on medium lacking a nutrient
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How do you detect a temperature sensitive mutant?
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Inability to grow at a high temperature that normally supports growth
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How do you detect a cold-senstivie mutant?
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Inability to grow at a low temperature that noramlly supports growth
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How do you detect a drug resistant mutant?
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Growth on a medium that normally inhibits growth due to drug concentration
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How do you detect a rough colony?
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Granular, irregular colonies instead of smooth, glistening colonies
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How do you detect a nonencapsulated org?
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Small, rough colonies instead of large smooth ones
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How do you detect a nonmotile org?
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Compact instead of flat spreading colonies
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How do you detect a pigmentless org?
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Presence of different color or lack of color
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How do you detect sugar fermentation mutant?
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Lack of color change on agar containing sugar and a pH indicator
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How do you detect a virus resistant mutant? What might cause this mutation?
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Growth in presence of large amounts virus
could be caused by loss of virus receptor. |
