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UV causes damage how?
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Direct DNA damage by causing Thymine dimers (bridges between)
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Why are Thymine dimers damaging in protein synthesis
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DNA polymerase misreads Dimers and mispairs nucleotides with uncomplementary pairs. This causes harmful mutations
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Are all UV induced mutations lethal? Why?
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No. Only lethal if vital genes for survival are mutated.
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Why does amount of UV exposure time affect bacteriocidal rate?
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The more time, the greater the risk of dimers and subsequent mutation for proteins vital to survival
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UV light is used to destroy microbes where? (3)
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Air, Water, Surfaces
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Why is UV light harmful to eyes?
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It can damage our genes too
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What is the explanation of why some colonies of Serracia marcesens may still be present even after some UV exposure?
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They may be mutants and still able to function
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What is a mutation?
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An incorrect pairing of nucleotide sequence in a DNA strand
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Process by which all microbes are destroyed
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Sterilization
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Adjective: abilitiy to kill bacterial cells on a surface, object or media
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Bacteriocidal
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Adj: ability to inhibit growth or bacterial proliferation
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Bacteriostatic
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Can bacteriostatic substances be used on surgical instruments or media? Why?
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No. Bacteria may be only inhibited not killed
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Any bacteriocidal or bacteriostatic substance is considered to be an __________
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Antimicrobial
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Disinfectants and Antiseptics are both ___________
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Chemical Antimicrobials
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Chemical antimicrobial used on inanimate objects
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Disinfectants
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