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Proteins, carbohydrates, and lipids are the three major biochemical compounds of human metabolism. What is the element that distinguishes proteins from carbohydrate and lipid compounds?
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Nitrogen
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Proteins may become denatured when subjected to mechanical aqitation, heat, or extreme chemical treatment. How are proeitns affected by denaturation?
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Alteration in tetiary structure
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What is the basis fo the Kjeldahl technique for the determination of serum total protein?
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Quantification of the nitrogen content of proeitn
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When qualifying serum total poreitns, upon what is the intensity of the color produced in the biuret reaction dependent?
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Number of peptide bonds
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Which of the following reagents can be used to measure protien in cerebrospinal fluid?
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Coomassie brilliant blue
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Which disorder is not associated with an elevated proeiten level in cerebrospinal fluid?
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Hyperthyroiddism
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Which term describes a congential disorder that is characterized by a split in the albumin band when serum is subjeced to electrophoresis?
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Bisalbuminemia
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In what condition would an increased level of serum albumin be expected?
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Dehydration
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Identification of which of the following is useful in early stages of glomerular dysfunction?
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Microalbuminuria
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Which glycoproeitn binds with hemoglobin to facilitate the removal of hemoglobin by the reticuloendothelial system?
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Haptyoglobin
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In a healthy individual, which protien fraction has the greatest concentration in serum?
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Albumin
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Which of the following is an anionic dye that binds selectively with albumin?
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Bromcresol green
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Which total protein method requires copper sulfate, potassium iodide, in sodium hydroxide, and potassium sodium tartrate in its reagent system?
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Biuret
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Which of the following plasma proeiten is not manufactured by the liver?
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IgG
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There are 5 immunoglobulin class: IgG, IgA, IgM, IgD and IgE. Which globulin fraction do these immunoglobulins migrate electrophorectically?
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Gamma-globulins
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