Lab Practical

1-4

95 cards   |   Total Attempts: 188
  

Cards In This Set

Front Back
The maximum useful magnification for a light microscope is...
1000x
The 2 image forming lenses of a compound light microscope are...
Ocular and objective
Dyes are usually added to sections of biological specimens to increase...
Contrast
What kind of microscope do you use if observing living surface of a finger?
Scanning Electron Microscope (SEM)
What kind of microscope is used for observing a dye stained slide of a section of your finger?
Compound Light Microscope
What microscope is used for observing gold coated bacteria on a single cell of a finger?
Scanning Electron Microscope (SEM)
What microscope is used when observing a unstained section of a biopsy from a finger?
Compound Light Microscope
WHat microscope is used when observing a heavy-metal-stained very thin section of a finger?
Transmition Electron Microscope (TEM)
Forms a TV Like picture from a secondary electron signal, which is emitted from the surface points excited by a thin beam of electrons drawn across the surface in a raster pattern. Investigates fine structure of surface. (Usually dead specimens)
Scanning Electron Microscope
Increases resolving power by using electrons in a vacuum and magnetic lenses instead of light and glass lenses. Preservation of greater specimen detail allows for magnifications up to 1,000,000x or more (usually dead materials)
Transmission Electron Microscope
Converts phase differences in light to differences in contrast Observation of low contrast specimens (often Living)
Phase-Contrast and Nomarski Process
Four steps when using compound light microscope.
1. Carry it to and from lab table by grasping arm and supporting base. 2. Keep it upright 3. Remove dust cover 4. If it needs to be dusted, use lens paper only.
The amt that an object image is enlarged
Magnification
The extent to which detail in an image is preserved during the magnifying process
Resolving Power
Focusesradiation emanating from an objective to produce an image
LEns