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Cards In This Set
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Name 2 important factors affecting our ability to see and characterize bacterial cells.
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Lack of contrast to background and their small size
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What 2 things are stains composed of?
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A chromophore and a solvent
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Basic stains have what kind of charge, negative or positive?
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Positive
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Basic stains are positve, and are attracted to negative or positive cells?
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Negative, opposites attract
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What charge are bacteria?
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Negative so basic stains dye the cell
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Acidic stains are negative, and are attracted to negative or positive cells?
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Neither, they are repelled by the negatively charged bacterial cell.
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Acidic stains color what part of the cell?
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Acidic stains dye the background and leave the cells uncolored
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Name 3 broad categories of staining techniques.
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Simple, differential and structural
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Simple staining, uses how many stains and shows us what?
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Uses a single dye or stain and shows us cell shape (morphology) cocci and bacillus; also shows cell arrangements, (single cell, diplococci, chains etc.)
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How many stains does differential staining use and shows us what?
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Differential staining uses 2 or more stains and allows for identification of specific groups of organisms
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The Gram Stain and Acid-Fast stain are 2 examples of what kind of stain? |
Differential stain
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How many stains do Structural stains use and what are 2 examples?
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Uses multiple dyes, 2 examples are Endospore stain and Flagella stain. Used to stain a particular structural component
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Name the 3 shapes of Bacterial cells
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Coccus (spherical), bacillus (oblong-rod), Spirillum or Vibrio (curved)
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What kinds of basic stains can simple stains use?
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Safranin or methylene blue which stain all of the bacterial cells one color
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What are procedures that stain cells called?
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Direct stains
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