Lab 13 Pg 91 Staining Techniques: Endospore Stain

Lab

28 cards   |   Total Attempts: 188
  

Cards In This Set

Front Back
Bacteria are subject to their environment, which 2 Genera have found a way to protect themselves?
Bacillus (Aerobe)
Clostridium (Anaerobe)
Both are vegetative cells (actively metabolizing, they begin the process of sporulation and eventually form Endospores
What is an Endospore?
A survival structure formed within the bacterial cell that allows the cell to survive until environmental conditions improve
What does an Endospore contain?
Copy of the organisms chromosome, a little cytoplasm and a small amount of cell machinery.
What does the Spore coat contain?
Keratin, Protein, Calcium, dipicolinic acid adn peptidoglycan
What are vegetative cells stressed by?
Nutrient limitation, temperature changes, waste accumulation, all these things begin the process of sporulation
What does Sporulation begin with?
Replication of the bacterial chromosome and the spatial separation of the chromosomes in different regions within the cell's cytoplasm
What is separation of the chromosomes's accomplished by?
The formation of a wall or septum within the cell
Name the 2 regions of the Septum
Forespore-will eventually become the Endospore
Sporangium-Metabolically active and produces and deposits the compounds necessary for the completion of the spore coat layers
An Endospore that develops at the end of a cell is called what?
Terminal Endospore
An Endospore that develops in the middle a cell is called what?
Central Endospore
An Endospore that develops between the center and the end of a cell is called what?
Subterminal Endospores
When the Endospore matures and dies over time, the cell wall breaks down releasing the Endospore, what it it called now?
Just a Spore or a Free Spore
What is environmentally resistant and very difficult to destroy?
Endospore
What 4 things do Endospores resist?
Heat, Radiation, Dessication and Chemicals, all these properties make it difficult to stain
The properties of Endospores make it easy to use them for what?
Biological weapons like Anthrax