Immunology LAB Exam 1 Flashcards

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36 cards   |   Total Attempts: 188
  

Cards In This Set

Front Back
Neutrophils appearance
3
-nucleus segmented in 2-5 lobes
-granules present but stain poorly
-granules contain antimicrobial chemicals
Eosinophil appearance
3
-bi-lobed nucleus
-granules stain red
-granules contain antimicrobial chemicals and histamine
Basophil appearance
4
-smaller than other leukocytes
-bi-lobed nucleus or unlobed
-granules stain dark purple
-granules contain histamine and other chemicals
Monocyte appearance
3
-twice the size of red blood cells
-round or indented (horseshoe-shaped) nucleus
-cytoplasm visible
Small lymphocyte appearance
3
-about the size of red blood cells
-thin halo of cytoplasm surrounding large round nucleus
-includes B and T cells
Large lymphocyte appearance
3
-same size as monocytes and neutrophils
-slightly larger cytoplasm surrounding a large round nucleus
-natural kill cellls
Neutrophil fucntion
-phagocytosis and digestion of (usually) bacteria
Eosinophil function
-present in inflammatory reactions and immune response agaisnt some multicellular parasites (worms)
Basophil function
-participate in inflammatory response
Lymphocyte function
-active in specific acquire immunity (as T and B cells)
Monocyte fucntion
-phagocytosis (as macrophages)
ELISA
what it stands for and characterisitcs of it
-enzyme-linked immunosorbent assay
-highly versatile, sensitive, and quantitative technique that requires little equipment and for which critical reagents are readily available
6 distinct ELISA protocols
1. indirect ELISA
2. direct competitive ELISA
3. antibody-sandwhich ELISA
4. double antibody-sandwich ELISA
5. direct cellular ELISA
6. indirect cellualr ELISA
Indirect ELISA uses
2
-antibody screening
-epitope mapping
Direct competitive uses
2
-antigen screening
-detect soluble antigen