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Cards In This Set
| Front | Back |
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Neutrophils appearance
3 |
-nucleus segmented in 2-5 lobes
-granules present but stain poorly -granules contain antimicrobial chemicals |
|
Eosinophil appearance
3 |
-bi-lobed nucleus
-granules stain red -granules contain antimicrobial chemicals and histamine |
|
Basophil appearance
4 |
-smaller than other leukocytes
-bi-lobed nucleus or unlobed -granules stain dark purple -granules contain histamine and other chemicals |
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Monocyte appearance
3 |
-twice the size of red blood cells
-round or indented (horseshoe-shaped) nucleus -cytoplasm visible |
|
Small lymphocyte appearance
3 |
-about the size of red blood cells
-thin halo of cytoplasm surrounding large round nucleus -includes B and T cells |
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Large lymphocyte appearance
3 |
-same size as monocytes and neutrophils
-slightly larger cytoplasm surrounding a large round nucleus -natural kill cellls |
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Neutrophil fucntion
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-phagocytosis and digestion of (usually) bacteria
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Eosinophil function
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-present in inflammatory reactions and immune response agaisnt some multicellular parasites (worms)
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Basophil function
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-participate in inflammatory response
|
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Lymphocyte function
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-active in specific acquire immunity (as T and B cells)
|
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Monocyte fucntion
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-phagocytosis (as macrophages)
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ELISA
what it stands for and characterisitcs of it |
-enzyme-linked immunosorbent assay
-highly versatile, sensitive, and quantitative technique that requires little equipment and for which critical reagents are readily available |
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6 distinct ELISA protocols
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1. indirect ELISA
2. direct competitive ELISA 3. antibody-sandwhich ELISA 4. double antibody-sandwich ELISA 5. direct cellular ELISA 6. indirect cellualr ELISA |
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Indirect ELISA uses
2 |
-antibody screening
-epitope mapping |
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Direct competitive uses
2 |
-antigen screening
-detect soluble antigen |
