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What is hemosiderin?
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Granules of non-heme iron which have formed water-insoluble complexes with lipids, proteins, and carbohydrates.
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How do you make Perl's Incubating mix for the Perl's Prussian Blue stain?
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30 mL 2% potassium ferrocyanide
30 mL 0.2 N HCl
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How do you prepare the iron counterstain for the Perl's Prussian Blue stain?
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100 mL nuclear fast red
50 mL basic fuchsin (pararosaniline)
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What is the purpose of the Hgb H stain?
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To aid in the diagnosis of alpha thalassemia.
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What is the composition of Hgb H?
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A tetramer of Beta chains
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Why might Hgb F be quantified by alkali denaturation?
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To aid in the diagnosis of a thalassemia or hemoglobinopathy.
Hgb F is especially high in Hb SS (Sickle cell), unstable hgb, double heterozygous conditions (SC disease, SD, S-thal), and in some cases of aplastic anemia, leukemia and pregnancy.
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The Sickle Cell Screening Test:
Deoxygenated hemoglobin is insoluble in the presence of a _____ buffer solution and forms a turbid suspension.
____ is used to lyse the red blood cells.
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1. phosphate
2. saponin
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Explain the heat stability test.
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Unstable hemoglobins will frm a flocculent precipitate within one hour of incubation at 50 degrees celsius.
Lyse blood with H2O before incubation.
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In the visual fluoresence screening test for G6PD deficiency, blood is incubated with ____ and _____.
Drops of blood are removed at five minute intervals and spotted on a filter paper and viewed under long wave ultra violet light.
What fluoresces, the normal blood are the deficient blood?
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Incubate the blood with G6PD and NADP.
Normal blood will fluoresce.
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Drabkin's reagent is used in the determination of Hgb to convert Hgb to ____ and then to _____.
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Methemoglobin, cyanmethemoglobin
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What are some conditions that elevate the erythrocyte sedimentation rate?
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Lab conditions:
- Having a centrifuge on the same bench
- Heat
- Humidity
- Tube at an angle
- High anticoagulant to blood ratio
Patient conditions: Bacterial infx, multiple myeloma, polycythemia rubra vera
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What is the refractive index of coverslip glass and immersion oil?
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1.52
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Why is NaCl often a diluent in automated cell counters?
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It does not corrode glass and tubing, there is a smaller chance of precipitate forming and blocking the tubing, and it allows the particles to become charged so that they can be measured
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What does a left shift in an RBC histogram indicate?
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Microcytosis
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Finding Heinz bodies indicates:
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- Chemical poisoning
- drug intoxication
- G6PD deficiency
- Other hemolytic anemia
- Unstable hgb (ex Hgb Koln)
- Post splenectomy
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