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Genetic Engineering
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The transfer of genetic information to an organism to introduce a desirable trait
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Phenotypes
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Physical traits of an organism, inheritable.
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Genotypes:
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The genetic makeup of an organism
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Genetic information is on...
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Chromosomes, genes make up chromosomes, code for one trait
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Prokaryotic
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“Simplest”forms of life, Bacteria Cyanobacteria, Lack complexity of Eukaryotes
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Eukaryotic
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More Organized and complex, Organelles- membrane bound structures such as Nucleus, chloroplasts, mitochondria
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Mitosis
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Cell division to produce identical daughter cells
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Meiosis
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Cell division to produce gametes, haploid cells
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Totipotency
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All cells contain same genetic information
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DNA- review structure of molecule
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Semi-conservative Replication
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Transcription
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Make mRNA from DNA
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Translation
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At Ribosomes, use tRNA to make protein- review processes
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Control of Gene Expression- Transcription factors and enhancers, different types of promoters
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Promoter regions of genes- Enhancers and Transcription factors
DNA coiling- exposing gene for transcription- histone proteins Micro RNA’s Differential Splicing Promoter regions of genes can determine whether or not a gene is activated Enhancers and Transcription factors Enhancers: regions of DNA to which transcription factors bind Transcription Factors are proteins Regulatory genes code for transcription factors that activate other genes Promoters can be constituative- always on Promoters can become active at stages of development Can become active under certain environmental conditions Can be useful for genetic engineering |
Making and probing Genomic Library
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