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How do autotrophs attain energy?
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They use light energy to synthesize food molecules
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How does the extraction of energy from food happen?
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!) enzymes break large molecules into smaller ones (digestion)
2) other enzymes dismantle fragments extracting energy at each stage (catabolism)
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What is glycoysis?
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Conversion of glucose to pyruvate followed by cellular respiration (conversion of pyruvate to carbon dioxide and water in the presence of O2) which results in a release of large amounts of energy
glucose + oxygen --> carbon dioxide + water + energy(ATP)
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Know the chart for the Stages in the extraction of energy from foodstuffs
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How do cells synthesize ATP?
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Substrate-level phosphorylation
and chemiosmosis - the majority is made this way in the inner mitochondrial membrane -- requires O2 *The synthesis of ATP requires energy |
Generation of ATP by substrate-level phosphorylation
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Involves direct transfer of phosphate group to ADP from another molecule
-eg. phosphoenolpyruvate (PEP) contains a high energy phosphate bond (similar to ATP), which can be transferred enzymatically to ADP |
What is reduction?
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The gain of one or more electrons by an atom, ion, or molecule
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What is oxidation
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Loss of one or more electrons
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What is a redox reaction (oxidation-reduction)?
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The transfer of electrons
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Do oxidation and reduction always occur together?
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Yes!
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What does NAD+ serve as?
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An "electron shuttle"
-exists in 2 forms; oxidized NAD+ and reduced NADH + H+ -functions as a coenzyme with dehydrogenases (specific type of enzyme) -energy is harvested from glucose molecules in gradual steps using NAD+ |
Figure 9.3
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The Respiratory Chain
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ATP formation in the mitochondria results from the flow of electrons through this.
-NADH molecules transfer electrons to a series of membrane protein complexes (NADH Q reductase, cytochrome reductase and cytochrome oxidase) -in conjunction with electron transport down the respiratory chain, protons are pumped across the membrane from the matrix |
Chemiosmosis
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The energy of the proton gradient across the mitochondrial membrane is used to drive ATP synthesis
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ATP synthase
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Returns protons to the matrix coupled with the synthesis of ATP
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