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Nucleic acids first isolated in:
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1868 by Fredrick Miescher
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Bases that are pyrimidines
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Cytosine, uracil, thymine
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Bases that are purines
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Adenine, guanine
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Pentoses join to bases at which C and which N
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C1 on the pentose sugar to N1 on pyrimidines or N9 on purines
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What type of organism are we talking about when discussing the lac operon
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One cell bacteria,prokaryotes that use glucose for energy & if isnt present lac operon gets it from the environment
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What is positive control?
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Transcription occurs only if a regulator molecule directly stimulates RNA production
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What is negative control?
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Transcription occurs unless it is inhibited by a regulator molecule
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What is an inducible system
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Presence of substrate initiates gene expression
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What is a repressible system
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Presence of substrate turns off the gene expression
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What type of control is the lac operon under
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Positive and negative
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Regulator for positive control of lac operon
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CAP, cAMP complex
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Regulator for negative control of lac operon
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Protein from lacI
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What kind of system is the lac operon
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Inducible- induced by lactose
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What does the protein encoded by the lacI gene do?
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It binds with lactose or operator, it is a regulator for negative control, a repressor protein
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What does the protein encoded by the lacZ gene do?
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Galactosidase, it cleaves lactose into glucose and galactose
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