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GGTP is used to diagnose these diseases
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Hepatobiliary disorder, chronic alcoholism
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Enzyme activity assay for GGTP that measures absorbance of p-Nitroaniline at 405-420 nm
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Szaz assay
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Catalyzes the breakdown of starch and glycogen via α, 1-6 branching linkages
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Amylase
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Amylase is increased in this disease
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Acute pancreatitis
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Measures the disappearance of starch substrate (Starch-iodine comp. [dark-blue] ↓color intensity)
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Amyloclastic
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Measures the appearance of the product (Starch reducing sugars)
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Saccharogenic
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Measures the ↑ in color (Insoluble starch-dye soluble starch-dye fragments)
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Chromogenic
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Coupling of several enzyme systems to monitor amylase activity
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Continuous monitoring
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Salivary Amylase which is fast moving
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Ptyalin
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Pancreatic Amylase which is slow moving
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Amylopsin
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Hydrolyzes fats to produce alcohols and FA
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Lipase
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Lipase is an early and specific marker for this disease
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Acute pancreatitis
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Substrate for Tietz and Cherry Crandall methods in determining lipase
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50% olive oil or triolein
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Titrating agent for Tietz and Cherry Crandall methods in determining lipase
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0.4 NaOH
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Indicator for Tietz method in determining lipase
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Thymolphthalein + veronal
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