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3' end has what group?
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Hydroxyl group
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5' end has what group?
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Phosphate group
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What holds the base pairs together?
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Hydrogen bonds
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What are the purines
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Adenine and guanine
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What are the pyrimidines?
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Thymine and cytosine
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How many bonds are made between A and T?
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2 bonds
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How many bonds are made between C and G?
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3 bonds
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What is semiconservative repllication?
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New strand is 1/2 parent template and 1/2 new DNA.
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What did meselson and stahl discover?
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That conservative and dispersive replication was false, and that dna replicates semiconservatively.
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What is the role of helicase in DNA replication?
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Unwinds the DNA helix
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What is the role of the single stranded binding proteins?
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They stablaize the leading and lagging strand once the helicase unwinds the DNA
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What is the role of DNA polymerase III?
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It adds new complementary bases to the old strands after they havebeen unwinded.
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Nucleotides arrive in the form of nucleoside, with three phosphates. Where do they get the energy to bond.
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They have three phospates(ATP) and they arrive with their own energy.
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Which end of the DNA does the DNA polymerase three add to?
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3' end of a growing DNA strand
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What are the limits of DNA polymerase III?
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Can only add to the three prime end.
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