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What are the 3 classes of hormones?
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Peptides, steroids, amines
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What class of hormones does insulin or beta-HCG belong to?
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Peptides
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How/when are peptide hormones made?
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Made by using enzymes to cleave precursor units. Made in advance & stored in granules until needed.
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What class of hormones does testosterone, estrogen, aldosterone, cortisol, FSH belong to?
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Steroids
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What are steroid hormones composed of/made from?
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Lipids (cholesterol)
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Are steroid hormones stored in cells until needed?
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No storage, just made when required.
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What class of hormones does epinephrine belong to?
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Amines
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What are amine hormones made from?
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Tyrosine
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Where are peptide hormones made?
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Pituitary, pancreas, parathyroid, heart, stomach, liver, kidney
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Where are steroid hormones made?
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Adrenal cortex, gonads, placenta
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Where are amine hormones made?
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Adrenal medulla, thyroid
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How do non-steroid hormones trigger a response in a cell?
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Bind to PM receptor, trigger cascade (secondary msngers - CAMP, etc)
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How do steroid hormones trigger a response in a cell?
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Cross PM, bind to nuclear receptors, activate specific genes for transcription.
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What is the difference btw a heterogenous & homogenous immunoassay?
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Heterogenous requires washing
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What does nephelometry measure? turbidometry?
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Light scatter; decreased light transmission (or increased absorbance)
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