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(page 264 "damage to proteins and nucleic acids" why is damage to the protein important
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Because the three dimensional shape fo the protein is what allows it to function
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What two things happen when the nucleic acid of a cell is damaged
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Fatal cell mutations and protein synthesis is stopped
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Name microorganisms from most suseptible to least
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Enveloped viruses, gram-positive bacteria, nonenveloped viruses, fungi, gram-negative bacteria, active stage protozoa, cysts of protozoa,mycobacteria, bacterial endospores
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What makes mycobacteria so resistant
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They have a large amounts of waxy lipids in their membranes
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High level germicide
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Kills all pathogens including bacterial endospores
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Intermediate level germicide
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Kills fungal spores, protozoan cysts, viruses, and pathogenic bacteria
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Low level germicide
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Eliminate vegetative bacteria, fungi, protozoa, and some viruses
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What two environmental conditions that affect microbial death rates and the efficiency of antimicrobial agents
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Temperature and pH
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Do most antimicrobial agents work best with high or low heat
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High
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Chemical disinfectants are increased at what pH
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Alkaloids
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What else makes agents more effective
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High concentration and fresher solutions and longer exposure
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What are the efficiencies of agents compared to
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The efficiency of Phenol (phenol coefficient)
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A phenol concentration greater than one indicates
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That an agent is more effective than phenol
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The larger the ratio
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The greater the effectiveness
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Use dilution test
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Researchers dip metal cylinders into broth cultures of bacteria, dry, then rinse them. They are then put into a fresh medium and incubated.
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