Cell Biology Test 3

Which of the following are aspects of fluorescence-ctivated cell sorting? A) A laser is used to assign charge to specific cells B) The detector in the cell srter detects negatively charged cells. C) Cells are separated from each other by charge difference. D) Cells are passed through a laser in groups of one thousand at a time. E) all of the above

Tissue culture cell lines... A) can be grown on a solid plastic support as provided by a petri dish B) may reuire growth in the presence of an ECM protein like collagen C) are not often derived from tumors D) all of the above E) A and B only

True or False: Low speed centrifugation of cells that have been broken open (homogenized) is an effective way to pellet ribosomes and indwelling viruses.

Proteins can be separated by which of the following? A) charge B) hydrophobicity C) size D) affinity for a particular molecule E) all of the above

In SDS-PAGE, what chemical is used to reduce disulfide bonds between subunits of proteins? ___________

What technique identifies a specific protein from a separated mixture of proteins? ___________ _____________

Which of the following gel electrophoresis techniques is most often used o separate DNA segments or even chromosomes from one another that are hundreds of thosands to millions of base pairs in size? A) agarose gel electrophoresis B) pulsed-field gel electrophoresis C) polyacrylamide gel electrophoresis D) SDS-PAGE E) C and D only

Reporter genes... A) include actin and myosin B) can be used to indicate where a gene is expressed C) can be used to indicate when a gene is expressed D) all of the above E) B and C only

Of the following, which would require use of an electron microscope to visualize? A) viable animal cell B) globular protein C) mitochondria D) cell from a tobacco leaf E) all of the above

In light microscopy, which lens focuses light on the specimen? __________

A light microscopy technique that relies on out-of-phase wavesof light that have passed thrugh cells for visualization is called ____________ microscopy.

Fluorescence microscopy... A) can utilize a fluorescent dye attached to an antibody. B) makes use of a specific excitation of wavelength. C) typically involves detection of a specific emission wavelength D) all of the above E) A and C only

True or False: Coomassie blue is an organic dye used to visualize proteins in cells via light microscopy.

The use of a primary antibody followed by a secondary antibody coupled to a fluorescent dye or enzyme that reacts with a chemical substrate to create a colored precipitate describes which of the following? A) signal amplification B) indirect immunocytochemistry C) deconvolution D) confocal fluorescence microscopy E) A and B only

In the electron microscope what is the counterpart to the light microscope lens? __________