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Calvin cycle rxn typeoverall rxnCannot proceed w/ out..
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Light independent (dark rxn) CO2-->(CH2O)NADPH & ATP
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Calvin cycle stages
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Carbon FixationReductive stageCycle regeneration
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Stage 1Carbon fixation enzymeHydrolysis of unstable product to make..
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Rubisco: Ribulose-1,5-bisphosphate carboxyoxygenase 2 molecules of 3-phosphoglycerate
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Rubisco activity requiresMg held in place how
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Mg & CO2 By (-) charges of ASP/GLU
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Primary function of rubisco
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Catalyzes carbon fixation
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Inefficient intermediate
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2-phosphoglycolate
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Stage 23-phosphoglycerate gets..enzymes involvedall happens where?
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Phosphorylated & reduced to glyceraldehyde-3-phosG3PD, Aldolase & fructose-1,6-bisphosphatase in stroma
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Stage 3produced...enzymes
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5-C sugarTransketoalase & aldolase
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Overall rxn:
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F-6-P+2GAP+1DHAP+3ATP-->3-ribulose-5-P+3ADP
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To produce 1 molecule of glucose...Cost of single glucose
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Must complete 2 rounds of calvin cycle54 ATP equivalents (18 ATP & 12NADPH)
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CelluloseStarchSucrose
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Structural glucose in plantsStorage form of glucose, made in chloroplastsTransportable glucose, made in cytoplasm
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Regulation of rubisco by..
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Light & thioredoxin
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Light drives ATP & NADPH synthesis by..Activator of rubisco
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Pumping protons into lumen from stromaMg leaves lumen to balance influx of protons
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Thioredoxin coordinates
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Electron transport & carbon fixation
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Pentose phosphate pathwayPhases
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Oxidative generation of NADPH Synthesis of 5-C sugars
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