+
Related Flashcards
Related Topics
Cards In This Set
| Front | Back |
|
What are some ways to extend the life of a microscope bulb
|
1) don't touch bulb with fingers (get oil on bulb)
2) use a low voltage setting (about 3-5) 3) don't move microscope until bulb has completely cooled |
|
The higher the magnification on the microscope, the _____ the condensor setting
|
Higher
|
|
What position should the condensor be put in when storing a microscope
|
In the lowest position
|
|
What is the problem when you can clearly see a specimen on low power objective but can't see or focus on the high or oil objective
|
Most likely the problem is the slide was loaded on the wrong side. Remove the slide and turn it over (flip it over) and reload the slide back onto the stage
|
|
How do you calibrate a refractometer
|
It is calibrated by placing distilled water that is between 60-100 F onto the glass cover and calibrate the refractometer to zero
|
|
What are the three layers of a spun hematocrit tube
|
Plasma/serum, buffy coat (WBC and platelets), RBC
|
|
What are the normal values for a feline
(PCV, TP, HGB, RBC and WBC) |
PCV- 30-45% (37%)
TP- 6.0-7.5 g/dL HGB- 12-15 g/dL RBC- 6.0-7.0 x 10(6)/mm(3) WBC- 5.5-19.5 x 10(3)/mm(3) |
|
What are the normal values for a canine
(PCV, TP, HGB, RBC and WBC) |
PCV- 37-55% (45%)
TP- 6.0-7.5 g/dL HGB- 12-15 g/dL RBC- 6.0-7.0 x 10(6)/mm(3) WBC- 6.0-15.0 x 10(3)/mm(3) |
|
What information can you obtain from a spun PCV
(5 things) |
PCV, TP, buffy coat, plasma color and microfalaria
|
|
If filling a hematocrit tube using blood straight from a syringe, what type of tube should you use
|
A heprin tube (red PCV tube)
|
|
If filling a hematocrit tube using blood from a purple top test tube, what type of tube should you use
|
A tube that doesn't of heprin in it (blue PCV tube)
- the purple top tube has heprin in (EDTA) so you don't need to use a hematocrit tube with heprin in it |
|
What is the difference between the red/tiger top tube and the purple/lavendar top tube
|
Red- used for getting serum
purple- has an anticoagulant, used for getting plasma |
|
What is the difference between serum and plasma
|
Serum- the liquid portion of clotted blood
plasma- the liquid portion of whole blood |
|
How long should a hematocrit tube be spun
|
-should be spun for 5 minutes
-make sure you place another tube of equal size/weight on the other side of the centrifudge to keep it balanced |
|
How do you measure TP (total protein)
|
Take a spun hematocrit tube, break it above the buffy coat and then using the serum/plasma portion, place a drop of it on the refractometer glass and look through the refractometer while pointing it towards light
|
