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Related Flashcards
Cards In This Set
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What are some reasons why we would obtain a bone marrow sample
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-anemias (non regen or persisten regen), - drug toxicities, - chemo patients, - neoplasia, - thrombocytopenia, - leukopenia, - tumors, - parasites, - abnormalities, - weird stuff, -you are looking for answers
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When should you reconsider taking a bone marrow sample
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In coagulopathies, it is contraindicated because it can cause the patient to bleed out, so do a clotting time test first
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What supplies do you need to perform a bone marrow aspirate
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-slides, -towel, - clippers, - sterile prep, -blade, - biopsy needle, - sterile drape, - sterile gloves, - EDTA, -syringe, - glue
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What type of anesthetic do doctors normally use for a bone marrow aspirate
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It depends on the doctor, they will either do a local or general anesthetic
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How do you prep the site for a bone marrow aspirate
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-the area is shaved , - surgically prepped, - aseptic technique is used
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What type of needle is most often used for bone marrow biopsy
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A pediatric jamshidi bone marrow biopsy needle
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What size syringe is normally used with a bone marrow aspirate
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A 12 ml syringe that is coated with EDTA
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Why do we perfer to have EDTA in our bone marrow syringe
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Bone marrow clots quickly
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How do you make EDTA to put in bone marrow syringe
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Put 1/2 ml of saline into a small EDTA tube and then it can be transfered to 3- 4 sm purple top tubes.... you will remove the needle and syringe to coat the syringe
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Why do we use EDTA over heparin when coating a bone marrow syringe
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EDTA is gentler on cells, heparin tends to distort cells
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What are some common sites used for a bone marrow aspiration
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-trochanteric fossa of proximal femur, - proximal humerus, - dorsal illiac crest/ wing of ilium, - sternum (used mainly in horses), - rib
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How do we make slides from a bone marrow aspirate
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-place drops onto a slide and lean slides against a towel to let contaminants fall to the bottom (blood), - then do a compression smear (do lots of smears)
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How do you obtain a bone marrow biopsy sample
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-when the bone marrow needle is advanced without the styletand then is removed from the bone. The sample is dislodged from the needle and placed in formalin.
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What should you never do when sending out bone marrow slides and biopsy for histopathology
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-never send the slides and biopsy togther, send seperatly. - the formalin can deterate the cells on the slide
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What is the griity material on a bone marrow slide
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Flecks of marrow
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