BIS104 Final

Isolate protein from background
using Ab and staphyloccus curease (protein A) which binds to Fc of IgG

1."humanized" anitbody which recognizers CD20 which is on all B cells on the immune system
2. Treats Non-Hodgken's Lymphoma

AMPIPATHIC (hydrophibic and hydrophilic)

1. phosphoglyceride
2. sphingolipids
3. cholesterol
4. carbohydrates

Head: stiffens the membrane
tail: kinks in unsaturated make them more liquid at room temperature

Seal all edges, pure sphere of H2O inside and out

1. liposomes: to seal all edges
2. self-aseembly in the cell
3. membranes are fluid
4. membranes have domians (ie. human sperm)
5. membranes have proteins

FRAP - GFP goes black to see if protein is dynamic or not

Adding hydrophobic lipid brings proteins ot the membrane after translation

Entirely extracytosolic
attached by noncovalent bonds, can use high salt solutino to strip them off

TRITON X-100
solubilizes membranes; preserve protein folding

SDS
denatures proteins, hydrophobic regions unfold by getting into hydrophobic parts of protein

digests proteins but can't cross membranes
(peripheral membrane proteins are degraded)

Extract by high pH which pokes holes in membrane

Inside: K

Outside: Cl-, Na+, Ca++

to pump out Na to keep cell regulated

From lumen: [low] --> Na+/glucose transporter to intestine cell

from intestine cell to blood: Na+/K+ ATPase to set up gradient, use facilitated transporter to get from [high->low]