Identify These Biology Lab BIM Reagent Tests Flashcards

Various Reactants and their Properties

14 cards   |   Total Attempts: 189
  

Cards In This Set

Front Back
Benedict's Solution
Test for Reducing Sugars like: Glucose or Fructose
Negative: Blue
Positive: Ranges from Green to Reddish Orange
Iodine
Test for Starch (due to coil shape of starch)
Negative: Yellowish Brown
Positive: Blueish Black
Sudan IV
Test for Lipid (reacts with Amino tail of chains)
Negative: doesn't mix (powder)
Positive: Red
Biuret
Test for Protein (Peptide Bonds of Protein chains)
Negative: Blue
Positive: Pink to Violet
Pepsin
Protein that breaks down Proteins into smaller amino acid chains.
Salivary Amylase
Enzyme that breaks down Starch to Glucose
Disch diphenylamine
Test for DNA (the dioxyribose sugar)
Negative: Clear
Positive: Blue (intensity indicative of amount of DNA)
Question 8
.
1. Positive (low amount)
2. Positive (medium amount)
3. Negative for reducing sugars
4. Positive for reducing sugars
5. Negative for reducing Sugars (Neg. Control)
6. Positive for reducing sugars (Pos. Control)
7. Negative for reducing sugars
Question 9
.
1. Negative for Starch
2. Positive for Starch
3. Negative for Starch
4. Negative for Starch
5. Negative for Starch (Neg. Control)
6. Negative for Starch
7. Positive for Starch (Pos. Control)
Question 10
.
1. Positive for starch (Pos. Control)
2. Negative for Reducing Sugar
3. Negative for Starch
4. Positive for Reducing Sugar** amylase has some sugar even though it is an enzyme
5. Negative for Starch
6. Positive for Reducing Sugar
7. Negative (Neg. Control)
8. Negative (Neg. Control)
Question 11
Biuret Test Tubes (for Proteins):
1) Egg Albumen
2) Honey
3) Amino Acid Solution
4) Distilled Water
5) Protein Solution
1. Positive for Protein
2. Negative for Protein
3. Negative for Protein
4. Negative for Protein (Neg. Control)
5. Positive for Protein (Pos. Control)
Biuret Test Tubes (Protein Digestion by Pepsin)
1) Distilled Water & B
2) Egg Albumen & B
3) Pepsin & B
4) Egg Albumen, HCL & B
5) Pepsin, HCL & B
6) Egg Albumen, Pepsin, HCL & B
1. Negative for protein/polypetide (Neg. Control)
2. Positive for Protein
3. Positive for Protein
4. Positive for Protein
5. Positive for Protein
6. Positive for Polypeptides (hydrolysis)
Sudan IV Test Tubes:
1) Salad Oil & Water
2) Salad Oil & Sudan IV
3) Honey & Sudan IV
4) Distilled Water & Sudan IV
5) Known Lipid & Sudan IV
1. Layers - Polar liquid, Non Polar liquid
2. Positive for Lipid
3. Negative for Lipid
4. Negative for Lipid (Neg. Control)
5. Positive for Lipid (Pos. Control)
Dische Diphenylamine Tests Tubes:
1) 2 ml DNA Solution
2) 1 ml DNA Solution 1 ml Water
3) Strawberry DNA Sample
4) 2 ml Distilled Water
1. Positive for DNA (large quantitiy)
2. Positive for DNA (Lesser quantity)
3. Negative for DNA*** (should be pos. /contaminated)
4. Negative for DNA (Neg. Control)