Front | Back |
Benedict's Solution
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Test for Reducing Sugars like: Glucose or Fructose
Negative: Blue Positive: Ranges from Green to Reddish Orange |
Iodine
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Test for Starch (due to coil shape of starch)
Negative: Yellowish Brown Positive: Blueish Black |
Sudan IV
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Test for Lipid (reacts with Amino tail of chains)
Negative: doesn't mix (powder) Positive: Red |
Biuret
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Test for Protein (Peptide Bonds of Protein chains)
Negative: Blue Positive: Pink to Violet |
Pepsin
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Protein that breaks down Proteins into smaller amino acid chains.
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Salivary Amylase
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Enzyme that breaks down Starch to Glucose
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Disch diphenylamine
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Test for DNA (the dioxyribose sugar)
Negative: Clear Positive: Blue (intensity indicative of amount of DNA) |
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1. Positive (low amount)
2. Positive (medium amount) 3. Negative for reducing sugars 4. Positive for reducing sugars 5. Negative for reducing Sugars (Neg. Control) 6. Positive for reducing sugars (Pos. Control) 7. Negative for reducing sugars |
. |
1. Negative for Starch
2. Positive for Starch 3. Negative for Starch 4. Negative for Starch 5. Negative for Starch (Neg. Control) 6. Negative for Starch 7. Positive for Starch (Pos. Control) |
. |
1. Positive for starch (Pos. Control)
2. Negative for Reducing Sugar 3. Negative for Starch 4. Positive for Reducing Sugar** amylase has some sugar even though it is an enzyme 5. Negative for Starch 6. Positive for Reducing Sugar 7. Negative (Neg. Control) 8. Negative (Neg. Control) |
Biuret Test Tubes (for Proteins): 1) Egg Albumen 2) Honey 3) Amino Acid Solution 4) Distilled Water 5) Protein Solution |
1. Positive for Protein
2. Negative for Protein 3. Negative for Protein 4. Negative for Protein (Neg. Control) 5. Positive for Protein (Pos. Control) |
Biuret Test Tubes (Protein Digestion by Pepsin)
1) Distilled Water & B 2) Egg Albumen & B 3) Pepsin & B 4) Egg Albumen, HCL & B 5) Pepsin, HCL & B 6) Egg Albumen, Pepsin, HCL & B |
1. Negative for protein/polypetide (Neg. Control)
2. Positive for Protein 3. Positive for Protein 4. Positive for Protein 5. Positive for Protein 6. Positive for Polypeptides (hydrolysis) |
Sudan IV Test Tubes:
1) Salad Oil & Water 2) Salad Oil & Sudan IV 3) Honey & Sudan IV 4) Distilled Water & Sudan IV 5) Known Lipid & Sudan IV |
1. Layers - Polar liquid, Non Polar liquid
2. Positive for Lipid 3. Negative for Lipid 4. Negative for Lipid (Neg. Control) 5. Positive for Lipid (Pos. Control) |
Dische Diphenylamine Tests Tubes:
1) 2 ml DNA Solution 2) 1 ml DNA Solution 1 ml Water 3) Strawberry DNA Sample 4) 2 ml Distilled Water |
1. Positive for DNA (large quantitiy)
2. Positive for DNA (Lesser quantity) 3. Negative for DNA*** (should be pos. /contaminated) 4. Negative for DNA (Neg. Control) |