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What is a microbiota?
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The types of bacteria growing on and in our bodies
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How is our body like an ecosystem?
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Different parts of our bodies have different measurements of pH, oxygen, relative humidities, temperatures, oiliness, UV exposures which combine together to select for different groups of organisms. This composition can change on different people
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How do we measure microbiota?
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Current methods include sequencing of ribosomal RNA genes or entire genomes to determine the diversity of microbiota
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What is a colony?
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A mound of millionsn of genetically identical cells
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How did cells grow on the agar plate?
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Wherever a single cell was deposited onto the agar, the cell will grow and divide repeatedly
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What is a lawn of bacteria?
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A dense even layer of bacteria
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What is a zone of inhibition?
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An area of clearing around the disc where sensitive organisms are unable to grow
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If zones in the culture are antibiotic resistant, what will you see?
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No zone of inhibition
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What are antibiotics?
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Antibiotics are substances that prevent or inhibit bacterial or fungal growth. They can have different specificities and different targets
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Where do antibiotics come from?
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Many of the first antibiotics that were discovered are produced by microorganisms in nature:
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What is antibiotic resistance?
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The ability to actively avoid the actions of an antibiotic
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Where are genes for antibiotic resistance found?
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On small circular DNA molecules called plasmids which can be shared between bacteria
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What consumes the majority of antibiotics in North America?
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Livestock in order to reduce disease in high disease density and as growth promoters
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What is tetracycline?
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A broad spectrum antibiotic that acts by preventing bacterial protein synthesis
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What is pencillin?
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Generally used to treat Gram-positive bacteria. It acts by inhibiting crosslinking in the bacterial cell wall
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