+
Related Flashcards
Related Topics
Cards In This Set
| Front | Back |
|
What functions do cell membranes have?
|
|
|
How can you study cellular structure?
|
By disrupting one or more of its functions and examining the effects on the cell
|
|
What is the red pigment in beet cells called? Where is it located?
|
Betacyanin. Located in the cell's large central vacuole, surrounded by a membrane called the tonoplast
|
|
Why are we using a spectrophotometer?
|
To quantitatively measure the amount of betacyanin in solution
|
|
What are chromophores?
|
The molecules or parts of the molecules that absorb light
|
|
What happens when a chromophore absorbs light?
|
Its electrons become excited
|
|
What is an absorption spectrum?
|
A graph that shows the amount of light absorbed by a chromatophore at a number of wavelengths
|
|
What does an absorption spectrum illustrate?
|
It illustrates which wavelength of light is absorbed maximally
|
|
How does betacyanin maximally absorb light?
|
At the wavelength of 525 nm
|
|
Concentration is proportional to what?
|
Absorbance
|
|
How does the spectrophotometer work?
|
|
|
Readings taken by the spectrophotometer can be expressed in which ways?
|
|
|
Within a specific range, absorbance is what?
|
A linear function of solute concentration. This means that if solute concentration doubles, the absorbance value will also double
|
|
What is turbidity?
|
A measure of the degree to which particles in suspension scatter light rather than absorb it
|
|
What two things prevent light from being transmitted?
|
Light absorption and light scattering
|
