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Related Flashcards
Cards In This Set
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Why do some bacteria require oxygen and others don't?
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Oxygen may or may not be used by bacteria for the formation of ATP (energy)
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How many stages in Cellular respiration? Does it require oxygen?
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3 major stages: Glycolysis, Krebs cycle, Electron Transport Chain (ETC). It is an aerobic or anaerobic process.
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What is an anaerobic process that is much less efficient that aerobic cellular respiration?
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Fermentation. Which consists of 2 major stages: Glycolysis and fermentation stage.
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Aerobes (Oxygen requirement and metabolism)
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Requires oxygen for survival and cellular respiration. POS for Superoxide Dismutase, POS for Catalase, NEG for Peroxidase. (Cellular respiration)
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Anaerobes (Oxygen requirement and metabolism)
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Do not require oxygen, obtain energy from fermentation or anaerobic cellular respiration. (Fermentation)
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Facultative anaerobes (Oxygen requirement and metabolism)
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Contain the enzymes to carry out cellular respiration and fermentation depending on the presence or absence of oxygen. POS for Superoxide Dismutase, POS for Catalase, NEG for Peroxidase. (Both Cellular respiration and Fermentation)
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Aerotolerant anaerobes (Oxygen requirement and metabolism)
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Do not use oxygen but have the enzyme to detoxify it. POS for Superoxide Dismutase, NEG for Catalase, POS for Peroxidase. (Fermentation)
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Obligate anaerobes (Oxygen requirement and metabolism)
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Do not have oxygen detoxifying enzymes, and will not survive in an oxygen rich environment. NEG for Superoxide Dismutase, NEG for Catalase, NEG for Peroxidase. Fermentation)
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Microaerophiles (Oxygen requirement and metabolism)
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Carry out cellular respiration but can only tolerate small amounts of oxygen. (Cellular respiration)
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Why can oxygen be toxic to some cells (obligate anaerobes) bot not to others?
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Superoxide free radicals formed during aerobic cellular respiration is a toxic byproduct that can denature proteins and damage DNA.
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What are 2 genera of anaerobic bacteria that have medical importance?
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Clostridium sp and Actinomyces sp. Clostridium sp are spore forming bacteria that can cause diseases like tetanus, gangrene or botulism in their vegetative state. Actinomyces sp can form tumor like masses and damage the jawbone.
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How does one determine the oxygen requirements of bacteria using Brewer type anaerobic jar?
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The Brewer jar utilizes a special catalyst to remove oxygen. The catalyst produces hydrogen ions that will react with the oxygen in the jar producing water.
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How does one determine the oxygen requirements of bacteria using thioglycollate media?
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Based on the location the bacteria grow in the media will help identify the bacteria oxygen requirements. One of the most commonly used media in microbiology lab. Bacteria with different oxygen requirements can be grown in it.
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The 3 major ingredients of thioglycollate media and their purpose?
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The reducing agent decreases the amount of oxygen in
the tube. The media at the top contains high oxygen while the media at the bottom
contains no oxygen. (thioglycollate) (anaerobic indicator) (agar to localize the bacteria)
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Why is thioglycollate media one of the most commonly used liquid median in clinical labs?
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Bacteria with different oxygen requirements can be grown in it.
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