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What is the basic purpose of the Microcosm experiment?
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To test the effect of nutrients on lake phytoplankton (microscopic algae)
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What are the key principles of experimental design?
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Developing a hypothesis
Designing an experiment with controls, Designing an experiment with replication Randomizing treatments (so as to not introduce other variables - example; if the microcosms in the sunny part of the room were all treated) |
What kind of microscope do we use in Lab?
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Zeiss Compound microscope
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What are these parts? |
These are the answers |
What magnification is achieved with oil immersion?
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1000 x
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Why do objects under oil immersion appear sharper?
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Because oil has a much higher refractive index than air
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What is an optical section?
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A single plane of focus in an object
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Are protozoans multicellular?
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NO they are unicellular
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What is Elodea?
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Elodea is a freshwater plant native to North America. It plays an important role in the ecosystem of lakes
and ponds as it provides a refuge for small invertebrates and larval fish and amphibians. It also is a food source for ducks and beavers. Elodea is commonly used in aquaria because of its attractive leaves and ability to propagate easily. |
Talk about Elodea cell structure
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There are 2 layers of cells, one larger, one smaller. The edge of the leaf is a single layer, and THORN cells project outward. These thorn cells might not have as many chloroplasts, and the nucleus and nucleolus may be visible.
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Cytoplasmic streaming in a leaf?
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The cytoplasm of a cell is continously moving around, and you can see the chloroplasts moving. their movement will show the location of the vacuole.
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What effect does Iodine have when added to a cell slide?
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Kills the cell, but makes some structures more visible by reacting with starch, so you can see the pyrenoids (starch containing bodies) in the chloroplasts.
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What is this ? |
1. Plasma Membrane 2. Cell Wall 3. Chloroplast 4. Vacule 5.Nuclear membrane 6. nucleolus 7. nucleus 8. cytoplasm 9. Elodea thorn cell and parts of adjacent cells |
What is a material safety data sheet?
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A document containing information about a controlled substance, namely toxic effects
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Why would you use more than one dye on a slide specimin?
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Because different dyes bond to different structures, so for example, green might show you cellulose, red might show you lignified cell walls, and purple might show starch grains
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